Coding
PhyB-642

Part:BBa_K365003:Design

Designed by: LACOTTE Yohann & ZIEGLER Thea   Group: iGEM10_ESBS-Strasbourg   (2010-09-14)

Phytochrome B from A. thaliana (aa 1-642)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 490
    Illegal BamHI site found at 572
    Illegal XhoI site found at 523
    Illegal XhoI site found at 542
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 739


Design Notes

To create the BioBrick part the sequence was amplified with primers containing the standard prefix with ATG and the fusion suffix of the Fusion Protein Assembly Standard.

Forward primer (5’->3’): (42 bp)
GGATCCgaattcgcggccgcttctagATGGTTTCCGGAGTC

Reverse primer (5’->3’) : (53 bp)
CAGCTGctgcagcggccgctactagtattaaccggtCCCCGCCATATCCCTAC


Source

The plasmid containing the PhyB-sequence was provided by the laboratory of Wilfried Weber from the University of Freiburg.

References

Voigt, C. A., A. Levskaya, et al. (2009). "Spatiotemporal control of cell signalling using a light-switchable protein interaction." Nature 461(7266): 997-1001.